TABLE 2.
Sensitivity of state-of-the-art analysis of SPMs using LC-MS/MS.
| Laboratory | References | Instrument LLOQ | LLOQ plasma/serum/fluid | Instrument | LLOQ definition | |
|---|---|---|---|---|---|---|
| pg on column | pg/ml vial | pg/ml | ||||
| Dalli | Colas et al. (2014) | 0.05–0.22 a , c , d5-LXA4 0.05, d5-RvD2, 0.09 | 1.3–5.5, LXA4 1.3, RvD2 2.3 | 0.05–0.22, d5-LXA4 0.05, d5-RvD2, 0.09 | Sciex 6500 | |
| Dalli | Gomez et al. (2020), Koenis et al. (2021), 10.21203/rs.3.pex-1147/v1 | 0.05–5.00, LXA4 0.1, RvD2 0.1 b , c | 1.4–142, LXA4 2.9, RvD2 2.9 | 0.05–5.00, LXA4 0.1, RvD2 0.1 | Sciex 5500 | |
| Dennis | Dumlao et al. (2011), Deems et al. (2007), Quehenberger et al. (2011) | 1 a , LXA4 1 | 25 | 2.8 | Sciex 4000 | S/N ≥ 3 (n = 3) (LOD) |
| Geisslinger | Toewe et al. (2018) | 1–2, LXA4 2, RvD2 2 | 100–200, LXA4 200, RvD2 200 | 25–50, LXA4 50, RvD2 50 | Sciex 5500 | S/N ≥ 10, ± 20% accuracy and precision |
| Giera | Jónasdóttir et al. (2015) | 0.5, LXA4 0.5, RvD2 0.5 | 25, LXA4 25, RvD2 25 | 200 | Sciex 6500 | S/N > 10 |
| Giera | Jonasdottir et al. (2017), Giera et al. (2012) | 0.4, LXA4 0.4 | 10 d , LXA4 10, RvD2 no calibration | Synovial fluid 12 | Sciex 6500 | S/N > 10 |
| Hammock | Yang et al. (2009) | 0.21 | 21 | 8 | Sciex 4000 | S/N ≥ 10 |
| Hersberger | Hartling et al. (2021) | 0.002–0.063, LXA4 0.008, RvD2 0.002 | 0.2–6.3, LXA4 0.8, RvD2 0.2 | 0.4–12.5, d5-LXA4 0.4, d5-RvD2 3.2 c | Sciex 6500+ | S/N > 10 |
| Mori | Mas et al. (2012) | 6 | 250 | 25 | Thermo TSQ Quantum | S/N ≥ 10 |
| Newmann | Pedersen et al. (2021) | 0.2–1, LXA4 0.4, RvD2 0.8 | 40–199, LXA4 80, RvD2 159 | 201–995, LXA4 398, RvD2 794 | Sciex 6500 | 3 × t n-1,0.95 × STD e |
| Nicholson | Wolfer et al. (2015) | 0.05–5, LXA4 1.3, RvD2 0.5 | 10–1000, LXA4 260, RvD2 100 | 12–1200, LXA4 312, RvD2 120 | Waters Xeno TQS | S/N > 5, intraday RSD <20% (n = 4), accuracy ±20% |
| Ramsden | Yuan et al. (2018) | 1–5, LXA4 2, RvD2 5 | 100–500, LXA4 200, RvD2 500 | 20–100, LXA4 40 RvD2 100 |
Sciex5500 | S/N > 5, intraday RSD <20% (n = 4), accuracy ±30% |
| Schebb | Kutzner et al. (2019) | 0.6–3.6, LXA4 0.6, RvD2 1.4 | 61–360, LXA4 61, RvD2 141 | 6–36, LXA4 6, RvD2 14 | Sciex 6500 | S/N ≥ 5, ±20% accuracy |
| Werz | Werner et al. (2019), Werner et al. (2020) | 0.195–1.56, LXA4 0.195, RvD2 1.56 a | 19.5–156, LXA4 19.5, RvD2 156 a | 1–8, LXA4 1, RvD2 8 a | Sciex 5500 | S/N > 3, >5 data points (LOD) |
| Zhu | Wang et al. (2020) | 0.18–4.5, LXA4 0.18, RvD2 0.9 | 1.8–45, LXA4 1.8, RvD2 9 | 5.4–135, LXA4 5.4, RvD2 27 | Agilent 6470 | S/N > 7 |
The reported sensitivity for all covered SPMs and exemplary LXA4 and RvD2 is summarized. Shown is the lower limit of quantification of the instrument (LLOQ on column and the corresponding concentration), the effective LLOQ for liquid biological samples in pg/ml, as well the instrument and method used for definition of the LLOQ during method validation. Please note, that this is a simplified table. An extended version of the table including all required information and more method parameters is provided in the supplemental information (Supplementary Table S1).
Only LLOD is given in the publication.
Not specified between LLOD/LLOQ.
Determined in plasma matrix.
Lowest calibration level injected.
LLOD/LLOQ was determined based on a significant change (one-tailed t-test) in the sensitivity between successive calibration standards using the standard deviation (STD) of the concentration level significantly different than the preceding concentration level and the t-distribution (t-value: one-tailed, 95% confidence).