FIGURE 1.
Expression patterns of E3 ubiquitin ligase genes in response to infection by M. grisea and hormone treatment. (A) Expression of E3 ubiquitin ligase genes in response to M. grisea. Leaves of H8R and H8S seedlings were sprayed with a solution of M. grisea spores. (B) Expression of E3 ubiquitin ligase genes in response to signaling hormones. Leaves of 4-week-old Yuanfengzao seedlings were treated with 1.5 mM SA, 100 μM JA, and 100 μM ACC solutions by spraying. JA and ACC solutions were prepared in 0.1% ethanol, and SA solution was prepared in water. The same volume of 0.1% ethanol or sterile distilled water was used as control. The samples were harvested at specific time points for the analysis of gene expression. Expression levels were presented as multiples of the OsActin expression level, which was used as standard. Data presented are the means ± SD from three independent experiments, and * above the columns indicate significant differences between the treated plants and control at p < 0.05 level.
