Fig. 5.

Role of heme oxygenase-1 (HO-1) in M2-polarized bone marrow-derived macrophages (BMDMs) engulfing apoptotic colonic epithelial cells. CD206 expression in BMDMs mixed with apoptotic epithelial cells in two ways to examine the role of HO-1 in vitro. (A) Murine BMDMs were treated with a pharmacological HO-1 inhibitor, zinc protoporphyrin IX (ZnPP) (10 mM), 1 hour prior to the addition of apoptotic epithelial cells. After incubation for 48 hours, the proportion of BMDMs expressing CD206 was measured by flow cytometry. (B) BMDMs from HO-1 knockout (KO) mice were treated with apoptotic epithelial cells and subjected to flow cytometry. Data are expressed as the mean±SEM (n=3).

WT, wild type; SEM, standard error of the mean. *p<0.01, ^†p<0.001.