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. 2022 Jan 7;23(3):743–759. doi: 10.1021/acs.biomac.1c01166

Figure 2.

Figure 2

Solubilization of the membrane protein, KcsA, from E. coli membranes by R-SMA (A), X-SMA (B), and R-SAA (C) copolymers. The general scaffolds are circled on the right with deviations from styrene marked in red and depicted above the bars and deviations from the maleic acid moiety marked in blue. Insets on the right show representative SDS-PAGE lanes for selected polymers (for complete overview see Figure S10) with the dashed box highlighting the band corresponding to the KcsA tetramer. M, molecular weight marker; S, supernatant; P, pellet. Solubilized KcsA (%) determined from the density of the KcsA band in S relative to the sum of S plus P. Data points indicate the mean ± SD (n ≥ 3 independent experiments). Densitometry data were obtained 2 h post-solubilization at 25 °C with a polymer concentration of 0.25% (w/v) (polymer-to-lipid ratio of ∼2.3:1 (w/w)).