Figure 4.

Whole membrane solubilization as determined by turbidimetry for E. coli (A–C) and yeast mitochondria (G–I) and the correlation with membrane protein (KcsA) extraction as determined by SDS-PAGE densitometry (D–F and J–L). The percentage of membrane solubilized is based on the relative decrease in optical density after 14 min (data are the average of 2 independent experiments ± range). Solubilization data are shown for analogues of R-SMA (left panels), X-SMA (middle panels), and R-SAA (right panels). Dashed lines indicate the line of linear fit, and the corresponding correlation coefficients (R²) are given in the left upper corner. For the complete turbidimetry traces, see Figure S11.