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. 2022 Mar 14;3(1):e31536. doi: 10.2196/31536

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©Martina Rueca, Emanuela Giombini, Francesco Messina, Barbara Bartolini, Antonino Di Caro, Maria Rosaria Capobianchi, Cesare EM Gruber. Originally published in JMIR Bioinformatics and Biotechnology (https://bioinform.jmir.org), 14.03.2022.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work, first published in JMIR Bioinformatics and Biotechnology, is properly cited. The complete bibliographic information, a link to the original publication on https://bioinform.jmir.org/, as well as this copyright and license information must be included.

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Classification scheme for genome assemblers, in which assembled genome sequences (SEQ) were compared with the corresponding submitted sequences (on GISAID) and with reference genome sequence “Wuhan-Hu-1” (REF). Nucleotide threesomes were classified using the following 11 categories: false deletion (Fd), false insertion (Fi), false negative (FN), false positive (FP), mutation error (Me), N correct (Nc), N error (Ne), true deletion (Td), true insertion (Ti), true negative (TN), true positive (TP).