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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: ACS Appl Mater Interfaces. 2021 Aug 17;13(34):40214–40228. doi: 10.1021/acsami.1c03504

Figure 4. In vitro PAM of targeted GNR in rabbit model of laser-induced choroidal neovascularization (CNV):

Figure 4.

(a) Color fundus photograph of the rabbit retina. The color fundus shows the retinal vessels (RVs) and optic nerve. (b–d) Fluorescein angiography (FA) images acquired at different time points after I.V. injection of fluorescein sodium: (b) early phase, (c) middle phase, and (d) late phase. The FA shows the morphology of retinal vessels, capillaries, and the location of CNV (white arrows). Red rectangle shows the selected scanning area. (e–k) Corresponding PAM images along the selected area outline in figure d obtained at two different excitation wavelengths of 578 and 700 nm. (e) 3D volumetric PAM visualization image (post 1 h). See Supplementary Visualization 2 for video representation. (f–h) Horizontal (x–y) MIP PAM images (post 1 h). (i–k) Vertical (y–z) PAM images (post 1 h). Pseudo yellow color represents the accumulation of GNRs in CNV. Noted that GNRs were laid under retinal vessels and above the choroidal vessels (k). (l) Sequential longitudinal in vivo PAM images (overlaid 3D images acquired at 578 and 700 nm) acquired on the same rabbit at various time points post administration of targeted GNR-RGD (0.4 mL, 2.5 mg/mL) and followed sequentially for 28 days.