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. 2022 Mar 2;154(4):e202112965. doi: 10.1085/jgp.202112965

Figure 4.

Figure 4.

Functional effect of the sFab on the voltage-gated Kv1.2–Kv2.1 chimera channel (KvWT) and on a construct in which the Kv1.2–Kv2.1 turret was substituted by that of KcsA (KvKcsA). (A) Sequence alignment of the pore domain of the Kv1.2–Kv2.1 channel with that of the KcsA channel. The turret interacting with the sFab is indicated by a dashed box. (B–E) Macroscopic currents of the full-length KvWT channel and of the KvKcsA construct recorded in Xenopus oocytes using pulses from −60 to +70 mV at 10 mV increments to test the functional impact of the sFab (n = 10). (B and C) KvWT current without sFab (B) and with 8 µM sFab (C). (D and E) KvKcsA construct current without sFab (D) and with 8 µM sFab (E). (F) I–V curve for the different experiments showing the effect of sFab on the conductance of the KvWT and KvKcsA channels.