KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-cyclin D1 mAb [SP4] | Abcam | Cat#ab16663; RRID: AB_443423 |
| Mouse anti-p27 mAb [SX53G8.5] | Cell Signaling Technology | Cat#3698; RRID: AB_2077832 |
| Rabbit anti-phospho-Rb (Ser807/811) mAb [D20B12] | Cell Signaling Technology | Cat#8516; RRID: AB_11178658 |
| Rabbit anti-phospho-Rb (Ser807/811) mAb (Alexa Fluor 488 conjugate) [D20B12] | Cell Signaling Technology | Cat#4277; RRID: AB_2797605 |
| Mouse anti-N-cadherin mAb [H-2] | Santa Cruz Biotechnology | Cat#sc-393933; RRID: AB_2832921 |
| Mouse anti-HA tag mAb [16B12] | Abcam | Cat#ab130275; RRID: AB_11156884 |
| Normal Rabbit IgG antibody | Cell Signaling Technology | Cat#2729; RRID: AB_1031062 |
| Chemicals, peptides, and recombinant proteins | ||
| PD0325901 | Selleck Chemicals | Cat#S1036; CAS#391210-10-9 |
| SCH772984 | Selleck Chemicals | Cat#S7101; CAS#942183-80-4 |
| LY3009120 | Selleck Chemicals | Cat#S7842; CAS#1454682-72-4 |
| PD-0332991 (palbociclib) | Selleck Chemicals | Cat#S1116; CAS#827022-32-2 |
| Cdk1/2 Inhibitor III | Millipore Sigma | Cat#217714; CAS#443798-55-8 |
| Doxycycline hyclate | Sigma-Aldrich | Cat#D9891; CAS#24390-14-5 |
| Bovine plasma fibronectin | Sigma-Aldrich | Cat#F1141 |
| Bovine collagen (type I) | Advanced BioMatrix | Cat#5005 |
| Critical commercial assays | ||
| ViewRNA ISH Cell Assay Kit | Thermo Fisher | Cat#QVC0001 |
| iTaq Universal SYBR Green Supermix | Bio-Rad | Cat#1725122 |
| Experimental models: Cell lines | ||
| Human: RPE-1 hTERT | ATCC | Cat#CRL-4000; RRID: CVCL_4388 |
| Human: MCF10A | ATCC | Cat#CRL-10317; RRID: CVCL_0598 |
| Oligonucleotides | ||
| Non-targeting siRNA | Dharmacon | Cat#D-001810-10-05 |
| CCND1 siRNA: 5′-GCAUGUAGUCACUUUAUAA-3′ | Dharmacon | Cat#J-003210-17 |
| CCND1 siRNA: 5′-GCGUGUAGCUAUGGAAGUU-3′ | Dharmacon | Cat#J-003210-18 |
| CDKN1B siRNA: 5′-ACGUAAACAGCUCGAAUUA-3′ | Dharmacon | Cat#J-003472-07 |
| CCND1 RNA FISH probe | Thermo Fisher | Cat#VA1-11978 |
| CDKN1B RNA FISH probe | Thermo Fisher | Cat#VA1-12174 |
| RPL10RNA FISH probe | Thermo Fisher | Cat#VA1-12078 |
| Pre-CCND1 RT-qPCR forward primer: 5′-TTTGTCATCGGCCAGAAATA-3′ | This manuscript | N/A |
| Pre-CCND1 RT-qPCR reverse primer: 5′-GACCTTCAGAGCACAGACCA-3′ | This manuscript | N/A |
| Pre-CDKN1B RT-qPCR forward primer: 5′-CGCAGGAATAAGGAAGCGAC-3′ | This manuscript | N/A |
| Pre-CDKN1B RT-qPCR reverse primer: 5′-GAATACGCCGAAAAGCAAGC-3′ | This manuscript | N/A |
| EEF1A1 RT-qPCR forward primer: 5′-GATGGCCAGTAGTGGTGGAC-3′ | This manuscript | N/A |
| EEF1A1 RT-qPCR reverse primer: 5′-TTTTTCGCAACGGGTTTG-3′ | This manuscript | N/A |
| Recombinant DNA | ||
| pLV-EF1a-H2B-mTurquoise-IRES-PuroR | Spencer et al., 2013 | N/A |
| CSII-hDHB (amino acids 994-1087)-mVenus | Spencer et al., 2013 | N/A |
| CSII-pEF1a-mCherry-Geminin (amino acids 1-110) | Cappell et al., 2016 | N/A |
| pLV-EF1a-CaaX-iRFP-IRES-Puro | Bisaria et al., 2020 | N/A |
| pPBbsr2-EKAR-NLS | Yang et al., 2017 | N/A |
| ERK KTR | Regot et al., 2014 | N/A |
| pCW-TRE-PGK promoter-cyclin D1-PuroR-T2A-rtTA | This manuscript | N/A |
| pCW-TRE-PGK promoter-p27-BlastR-T2A-rtTA | This manuscript | N/A |
| pCW-TRE-PGK promoter-HA-MEK1-S218D/S222D-PuroR-T2A-rtTA | This manuscript | N/A |
| Software and algorithms | ||
| Microscopy image analysis: cell tracking | Cappell et al., 2016 | https://github.com/scappell/Cell_tracking |
| Microscopy image analysis: custom code for measuring local cell density | This manuscript | Zenodo: https://doi.org/10.5281/zenodo.4960626 |
| Other | ||
| Sylgard Silicone Elastomer Kit 184 | Dow | SKU#DC4019862 |