Fig. 1.
Experimental procedure. Three-month-old AppNL-G-F mice were assigned randomly into the vehicle and probiotic groups: the vehicle group (n = 26) received saline and the probiotic group (n = 26) was supplemented with B. breve MCC1274 (1×109 cfu/5.56 mg/200μl saline/mouse) via oral gavage five times/week for four months. At the end of supplementation, feces were collected, and then the mice were behaviorally evaluated by novel object recognition (NOR) test or injected with BrdU. The mice were sacrificed and brains collected for biochemical analyses and immunostaining.