Fig. 4.
B. breve MCC1274 supplementation does not affect the phosphorylation of tau and attenuates microglial activation in the hippocampus of AppNL-G-F mice. A) The protein levels of total Tau, AT180 (p-Thr23), PHF1 (p-Ser369/Ser404), and actin in the hippocampus were determined by western blot analysis, quantified by densitometry, normalized to actin level, and expressed as a value relative to the control. B) Brain sections were stained with the anti-Iba1 (red) and anti-Aβ (green) antibodies, and cell nuclei were stained with DAPI (blue). Representative images of the hippocampus (left panel). Highly magnified images of the squared region in the left panels are shown in the adjacent right panels. Numbers of Iba1-positive cells (right panel) in the hippocampus. Data are expressed as the mean±SD, n = 6-7, *p < 0.05 compared with the vehicle group, n.s., no significant difference as determined by Student’s t-test.