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Levels of serum MT (A), calcium (B), phosphorus (C), ALP (D), OCN (E), and gene expression levels of Runx2 (F) and Sp7 (G). n = 3 per group. Data represent means ± SEM. The single statistical markers above in the early and late groups were compared with the Sham + Ctrl groups. Unpaired t-tests were used to compare groups. ns represents no statistical difference, *P < 0.05, **P < 0.01, ***P < 0.001
