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. Author manuscript; available in PMC: 2022 Mar 16.
Published in final edited form as: Biochemistry. 2019 Nov 20;58(49):4983–4996. doi: 10.1021/acs.biochem.9b00863

Figure 7.

Figure 7.

A. Expression of VWA8 in knockout cells lowers respiration to wildtype levels. Average data (± SD) are shown for wildtype (closed circles), knockout (open circles), knockout with VWA8 rescue (closed diamonds), and knockout with empty adenoviral vector control (open diamonds). Palmitate was used as fuel. B. Quantification of superoxide production (n=12, **p<0.01 vs. Ad-Vec). C. Immunoblots showing rescue of VWA8a (Ad-VWA8a) or VWA8b (Ad-VWA8b) restores protein abundance of marker proteins to wildtype levels. D. Quantification of immunoblots (n=3). No antibody is available for VWA8b, so an HA tag was used CPS-1, carbamoyl phosphate synthase-1; MTCO2, mitochondrial cytochrome c oxidase subunit 2; SOD2, superoxide dismutase 2; Cyto-C, cytochrome C reductase. Statistical comparisons of hydrogen peroxide production were made using one-way analysis of variance.