TABLE 1.
FDA-cleared antimicrobial resistance gene markers detected by the Acuitas AMR gene panel for each bacterial species
| Organism | Reported AMR gene marker(s) |
|---|---|
| Citrobacter freundii complexa | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Citrobacter koseri | KPC, OXA-48 |
| Enterobacter cloacae complexb | CTX-M-1, CTX-M-9, KPC, TEMd |
| Enterococcus faecalis | vanA |
| Escherichia coli | AAC, ANT, CMY, CTX-M-1, CTX-M-2, CTX-M-9, DFR, gyrA mutantc, KPC, MCR-1e, OXA-1, OXA-9, SHVd, Sul1, Sul2, TEMd |
| Klebsiella aerogenes | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella michiganensis | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella oxytoca | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella pneumoniae | AAC, AAD, APH, CMY, CTX-M-1, CTX-M-9, DFR, DHA, IMP, KPC, NDM, OXA-1, OXA-9, OXA-48, RMT, Sul1, Sul2, TEMd |
| Klebsiella quasipneumoniae | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Klebsiella variicola | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
| Morganella morganii | CTX-M-1, KPC, NDM, OXA-48 |
| Proteus mirabilis | AAC, ANT, APH, armA, CMY, CTX-M-1, CTX-M-2, CTX-M-9, DFR, KPC, NDM, OXA-1, OXA-9, OXA-48, Sul2, TEMd, VEB, VIM |
| Providencia rettgeri | NDM |
| Providencia stuartii | NDM |
| Pseudomonas aeruginosa | AAC, ANT, CTX-M-1, CTX-M-2, gyrA mutantc, KPC, NDM, OXA-1, PER, SHVd, TEMd, VEB, VIM |
| Raoultella ornithinolytica | KPC, NDM, OXA-48 |
| Raoultella planticola | KPC |
| Serratia marcescens | CTX-M-1, CTX-M-9, KPC, NDM, OXA-48 |
Citrobacter freundii complex = C. freundii, C. braakii, C. werkmanii, and C. youngae.
Enterobacter cloacae complex = E. asburiae, E. cloacae, E. hormaechei, E. kobei, and E. ludwigii.
PCR assays associated with fluoroquinolone resistance detect and differentiate wild-type and mutant variants of DNA gyrase A at amino acid position 87 for E. coli and position 83 for P. aeruginosa.
PCR assays for SHV and TEM detect several sequence variants for the two genes, respectively, at amino acid positions 156 and 104 associated with wild-type penicillin resistance and mutations associated with ESBL phenotypes.
The panel includes an assay for the detection of the mobilized colistin genetic determinant MCR-1 in E. coli.