TABLE 2.
Patient data and carbapenem and ceftazidime-avibactam susceptibility of KPC-producing K. pneumoniae isolates and results of phenotypic methods for KPC detectiona
| Sample | Source | Date (day/mo/yr) | CAZ-AVI exposure at time of culture (days) | MIC values (mg/L) |
ChromID plates |
Phenotypic methods |
Rapid molecular methods |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IMP | MEM | CAZ/AVI | ESBL | CARBA SMART | IC |
β-CARBA test | Rosco Diagnostica |
DDST-ESBL | Eazyplex system | Xpert Carba-R | KPC variant | ||||||
| O.K.N.V.I. RESIST-5 | NG-test CARBA 5 | CP | ESBL/AmpC | ||||||||||||||
| A-1 | BAS | 02/03/2020 | +1 | 16 | 8 | 2 | + | + | + | + | + | + | −/– | − | + | + | KPC-3 |
| A-2 | Rectal | 25/03/2020 | +6 | ≤1 | ≤ 0.12 | 32 | + | − | + | + | − | − | +/– | + | + | + | KPC-46 |
| B-1 | Rectal | 02/06/2020 | 0 | 16 | >16 | 2 | + | + | + | + | + | + | −/– | − | + | + | KPC-3 |
| B-2 | Rectal | 16/06/2020 | +8 | ≤1 | ≤ 0.12 | 8 | + | − | + | + | − | − | +/– | + | + | + | KPC-66 |
| C-1 | Rectal | 09/06/2020 | 0 | 16 | >16 | 1 | + | + | + | + | + | + | −/– | − | + | + | KPC-3 |
| C-2 | Blood | 13/07/2020 | +24 | ≤1 | ≤ 0.12 | 8 | + | − | + | + | − | − | +/– | + | + | + | KPC-92 |
a
BAS, bronchial aspirate; IMP, imipenem; MEM, meropenem; CAZ/AVI, ceftazidime-avibactam; IC, immunochromatography test; CP, carbapenemases; DDST-ESBL, susceptibility testing by disk diffusion for ESBL detection; +, positive result for KPC carbapenemase detection, −, negative result for KPC carbapenemase detection.