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. 2022 Apr;28(4):583–595. doi: 10.1261/rna.078977.121

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© 2022 Maul-Newby et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

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FIGURE 4. — A^min destabilization does not occur via an extended PYT. (A) Schematic of the A^min RNA substrate with branch point sequence (BPS) and PYT. The arrows indicate the 3′ end of the PYT in each RNA. (B) Representative native gel analysis of in vitro spliceosome assembly with truncated A^min substrate ±ATP at the indicated timepoints. PYT length is indicated on the right. (C) Background-corrected band intensity of A^min- and *-complexes from two independent trials. Graphs correspond to the adjacent native gel images in B.