Fig. 7. Enhanced in vitro antibacterial potency of high-DAR TXC. (A) Structures of αWTA/XTEN144-dmDNA3118 TXC (DAR = 18) and αWTA/dmDNA312 TDC (DAR = 2). (B and C) S. aureus USA300 bacteria were incubated with conjugates (TXC in blue or TDC in black) or free mAb (purple, used at 100 nM) and ingested by macrophages. After further incubation to enable intracellular killing, bacterial viability was assessed by enumerating colony-forming units (CFU). Anti-S. aureus activity was expressed as a function of molar concentration of (B) conjugate or (C) payload. LOD, limit of detection. Data represent average ±SD of 3 experiments.
