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Fluorescence activated cell sorter (FACS) analysis of enhanced green fluorescent protein (EGFP) expression. MC3T3-E1 (A) was transfected with empty plasmids (pEGFP) (B) or constructed osteoprotegerin (OPG) recombinant (pOPG) (C) and was selected by G418. FACS analysis showed that most of the stable transfection cells shifted to the right with EGFP expression compared with the nontransfected cells.
