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Osteoprotegerin (OPG) expression: (A) Secreted OPG protein was measured by enzyme-linked immunosorbent assay (ELISA). Data were the means of triplicates indicated by standard deviation (t-test; *P < .05). (B) Western blot for OPG protein in cytoplasm. OPG protein was confirmed to overexpress in MC3T3-E1.
