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Alkaline phosphate (ALP) expression in osteoprotegerin (OPG)-expressing cells (pOPG) and the control group (pEGFP). Cells were collected and lysed to subject enzyme-linked immunosorbent assay (ELISA) measurement. Data were the means of triplicates with the standard deviation bar. ALP activity in the OPG-expressing cells (pOPG) was significantly higher than in the control group (pEGFP) (t-test; **P < .01).
