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. 2022 Mar 4;11:e73093. doi: 10.7554/eLife.73093

Figure 2. Global conformational changes of hHv1 channels driven by voltages.

Figure 2.

FRET histograms and state occupancies were calculated from the single-molecule fluorescence resonance energy transfer (smFRET) data at the S98C-Q194C, Q194C-S219C, S98C-N132C, and K125C-K169C labe−ling sites on the N214R background. All data were analyzed with the four state kinetic model as shown in Figure 1F, except for the K125C-K169C sites with a three state kinetic model containing F0.66, F0.4, and the FB state for bleaching/blinking events. The state occupancy data presented as mean ± SE, N is the number of smFRET traces. Unpaired t-tests were performed to examine the significance levels of voltage-induced FRET state occupancy changes, in comparison to the occupancy of the same FRET state at −85 mV, but none of these changes reached the significant level of p<0.05.