TABLE 2.
Oligonucleotides for detection and amplification of blaOXY regions
| Primer | Sequence | Positiona | Reference (accession no.) |
|---|---|---|---|
| C | 5′-GCGTAGCGCTGATTAACACG-3′ | 494–513 | 9 (Z30177) |
| D | 5′-CCTGCTGCGGCTGGGTAAAA-3′ | 1143–1162 (comp) | 9 (Z30177) |
| L | 5′-CAGATCTCGAGAAGCGTTCA-3′ | 421–440 | 11 (Z49084) |
| M | 5′-ACCTCTTTGCGGTTTTTCGC-3′ | 1125–1144 (comp) | 11 (Z49084) |
| KHUBI | 5′-CCAGGATCCTGGCGAGACACTATAAGC-3′b | 174–193 | 11 (Z49084) |
| KHDBI | 5′-CCTGGATCCAGAGTGCAGAGTGTTGCA-3′ | 994–1012 (comp) | 13 (D84548) |
| CYC7BI1 | 5′-CAAGGATCCAGGTGAAGATCCT-3′ | 680–701 | 19 (X06402) |
| CYC7BI2 | 5′-TCGGGATCCACCAGAATGACATC-3′ | 3133–3158 (comp) | 19 (X06402) |
a
Comp, complementary strand of DNA sequence.
b
Underlined sequences indicate BamHI restriction sites.