Figure 4. Fbw7 exhibits mutation-type specific regulation and coordinate regulation of multiple TFs.

(A) The overlap between peaks with increased Jun in Fbw7^−/− and Fbw7^R/+ cells. (B) Genome browser view of Jun and H3K27ac occupancy in Hct116 WT, Fbw7^−/−, and Fbw7^R/+ cells at representative loci. Black arrows point to peaks with increased signal uniquely in Fbw7^−/− (KCNQ5), in both Fbw7^−/− and Fbw7^R/+ (intermediate level in Fbw7^R/+) (ITGA2) and increased in Fbw7^R/+ than in Fbw7^−/− (MAML2). (C, D) Heatmap of Jun and H3K27ac signal from each cell type mapped on sites with increased Jun in Fbw7^−/− cells. (E–H) (E) the overlap between peaks with increased Jun and Myc, (F) decreased Jun and Myc, (G) increased Jun and decreased Myc, and (H) decreased Jun and increased Myc in Fbw7^−/− cells. See Figure 4—figure supplement 1. TF, transcription factor; WT, wild-type.

Figure 4—figure supplement 1. Comparison between Jun and Myc peaks in Hct116 cells.

(A) The overlap between Jun and Myc peaks in Hct116 WT cells (p<0.0001, Fisher test). (B) The overlap between peaks with increased Jun occupancy in Fbw7^−/− and Fbw7^R/+ cells, and decreased Myc occupancy in Fbw7^−/− and Fbw7^R/+ cells. (C) The overlap between decreased Jun occupancy in Fbw7^−/− and Fbw7^R/+ cells, and increased Myc occupancy in Fbw7^−/− and Fbw7^R/+ cells.