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. 2022 Mar 17;207:114192. doi: 10.1016/j.bios.2022.114192

Fig. 2.

Fig. 2

Video processing algorithm. Python code was developed to automatically obtain the flow distance over time. (A) The red squares were detected, and each frame is rotated for orientation correction. (B) The green edge was recognized and cropped. (C) The cropped area was analyzed to generate an intensity histogram plot. Using appropriate thresholding, the liquid flow was recognized on paper. (D) The flow on each channel was read separately by recognizing it as the black pixels increasing along the vertical centerline. (E) Flow distance vs. time profile of mAb-particles on the preloaded 1000 fg/μL SARS-CoV-2 spiked sample. (F) Flow distance vs. time profile of pAb-particles on the preloaded 1000 fg/μL SARS-CoV-2 spiked sample.