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. 2022 Mar 17;207:114192. doi: 10.1016/j.bios.2022.114192

Fig. 3.

Fig. 3

Assay LOD and Specificity. NC indicates negative control and * shows p < 0.05 between sample and NC using one-tailed student's t-test with unequal variance. Error bars represent standard error. (A) Flow distances at 30 s on the paper microfluidic chips preloaded with SARS-CoV-2 spiked in 1% v/v human pooled saliva, using polyclonal antibody conjugated particles at 0.04 μg/μL (n = 3). (B) Flow distances at 30 s on the paper microfluidic chips preloaded with SARS-CoV-2 spiked in simulated saline gargle samples (∼15% v/v saliva and 0.9% saline), using polyclonal antibody conjugated particles at 0.04 μg/μL, with the addition of 0.5% w/v Tween 20 (n = 3). (C) Specificity test results with 1 pg/μL SARS-CoV-2 and influenza A/H1N1 (Ct values of 25–28) spiked in 1% v/v and 10% v/v saliva in 0.9% saline using the pAb-particles, shown together with the no target control samples (1% or 10% saliva in 0.9% saline) (n = 3).