Fig. 4. Protein alignment of ABCD1 orthologs and Sanger sequencing validation of the ABCD1 mutation. A: Protein alignment of seven ABCD1 orthologs showing conservation of histidine (in blue) from human to fruit fly. B: Sanger sequencing validation of the ABCD1 mutation. (a) Wild-type electropherogram. (b) Electropherogram of the two affected brothers. (c) Electropherogram of the mother.
