FIGURE 5.

The mitochondria released from neurons were taken up by astrocytes. (A) Experimental schematic to detect the cross-talk between neurons and astrocytes after treatment with pH 7.4 and 6.5 solutions. The neurons were labeled by MitoTracker Red CMXRos, and the astrocytes were labeled with GFAP. (B) The mitochondria with MitoTracker Red CMXRos were rarely detected within the astrocytes after co-culture with CM from pH 7.4-treated neurons. However, the co-localization of MitoTracker Red CMXRos with GFAP was increased when co-culture with CM from pH 6.5-treated neurons, and the increase was abolished by removing the mitochondria with a 0.2 μm filter.