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. 2022 Mar 3;14:785761. doi: 10.3389/fnagi.2022.785761

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Copyright © 2022 Gao, Liu, Hou, Manaenko, Xiao, Wang, Xu and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The detection of mitochondria and Δψm (JC-1 red/green ratio) in the CSF of MCAO mice. (A) After cerebral ischemia, the MitoTracker Green-labeled mitochondria elevated significantly in CSF at 24-h post-reperfusion. n = 8. *p < 0.05 vs. Sham. (B) The JC-1 staining in CSF showed no significant difference of JC-1 red between the Sham and MCAO groups. (C) JC-1 green increased significantly in the CSF after MCAO when compared with the Sham group. (D) JC-1 red/green ratio decreased significantly in the CSF after MCAO. n = 6. *p < 0.05 vs. Sham. CSF, cerebrospinal fluid; MCAO, middle cerebral artery occlusion; n.s., not significant.