REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-GFP Polyclonal antibody	Thermo Fisher Scientific	Cat#A-11122 RRID;AB_221569
Mouse anti-GFP [9F9.F9] Monoclonal antibody	Abcam	Cat#ab1218; RRID:AB_298911
Rabbit anti-RFP Polyclonal antibody	Abcam	Cat#ab62341; RRID:AB_945213
Mouse anti-Bruchpilot Monoclonal antibody	DSHB	Cat# nc82; RRID:AB_2314866
Alexa Fluor 488 goat anti-mouse Polyclonal antibody	Abcam	Cat#ab150113; RRID:AB_2576208
Alexa Fluor 488 goat anti-rabbit Polyclonal antibody	Thermo Fisher Scientific	Cat#A-11034; RRID:AB_2576217
Alexa Fluor 568 goat anti-rabbit Polyclonal antibody	Abcam	Cat#ab175471; RRID:AB_2576207
Alexa Fluor 647 goat anti-mouse Polyclonal antibody	Abcam	Cat#ab150115; RRID:AB_2687948
Chemicals, Peptides, and Recombinant Proteins
All-trans retinal	Sigma-Aldrich	Cat#R2500; CAS: 116-31-4
Quinine hemisulfate monohydrate	Alfa Aesar	Cat#A17036; CAS: 6119-70-6
1-Hexanol	Sigma-Aldrich	Cat#H13303; CAS: 111-27-3
Ethyl benzoate	Sigma-Aldrich	Cat#E12907; CAS: 93-89-0
Ethyl acetate	Sigma-Aldrich	Cat#270989; CAS: 141-78-6
2-heptanone	Sigma-Aldrich	Cat#W254401; CAS: 110-43-0
Methyl acetate	Sigma-Aldrich	Cat#W267600; CAS: 79-20-9
2-butanone	Sigma-Aldrich	Cat#34861; CAS: 78-93-3
3-octanol	Sigma-Aldrich	Cat#W358126; CAS: 589-98-0
MCH (4-Methylcyclohexanol)	Sigma-Aldrich	Cat#153095; CAS: 589-91-3
Geranyl acetate	Sigma-Aldrich	Cat#W250902; CAS: 105-87-3
Ethyl lactate	Sigma-Aldrich	Cat#W244015; CAS: 97-64-3
Hexyl acetate	Sigma-Aldrich	Cat#108154; CAS: 142-92-7
Apple cider vinegar	Rauch Fruchtsäfte GmbH & Co OG	N/A
Mineral oil	Sigma-Aldrich	Cat#330779; CAS: 8042-47-5
Experimental Models: Organisms/Strains
D. melanogaster: GH146II-GAL4	BDSC	Cat#30026; RRID:BDSC_30026
D. melanogaster: GH146X-GAL4	Gift from Dr. Christopher J. Potter43	Cat#91812; RRID:BDSC_91812
D. melanogaster: GH146-QF, QUAS-mtdTomato	BDSC	Cat#30037; RRID:BDSC_30037
D. melanogaster: GH146-QF, QUAS-mCD8-GFP	BDSC	Cat#30038; RRID:BDSC_30038
D. melanogaster: GH146-QF	BDSC	Cat#30015; RRID:BDSC_30015
D. melanogaster: MDN3-GAL4: VT50660-p65ADZp (attP40); VT44845-ZpGAL4DBD (attP2)	Gift from Dr. Barry J. Dickson18,88	N/A
D. melanogaster: MDN+MAN-LexA: UAS-TNT-E, VT49484-ZpLexADBD (JK22C); VT50660-p65ADZp (attP2), 13XLexAop-CsChrimson-tdTomato (VK00005)	Gift from Dr. Barry J. Dickson21	N/A
D. melanogaster: w1118	BDSC	Cat#5905; RRID:BDSC_5905
D. melanogaster: Or82a-GAL4	BDSC	Cat#23125; RRID:BDSC_23125
D. melanogaster: Ir31a-GAL4	BDSC	Cat#41726; RRID:BDSC_41726
D. melanogaster: Ir40a-GAL4	BDSC	Cat#41727; RRID:BDSC_41727
D. melanogaster: Ir25a-GAL4	BDSC	Cat#41728; RRID:BDSC_41728
D. melanogaster: Ir76b-GAL4	BDSC	Cat#41730; RRID:BDSC_41730
D. melanogaster: Ir8a-GAL4	BDSC	Cat#41731; RRID:BDSC_41731
D. melanogaster: Ir64a-GAL4	BDSC	Cat#41732; RRID:BDSC_41732
D. melanogaster: Ir92a-GAL4	BDSC	Cat#41733; RRID:BDSC_41733
D. melanogaster: Ir84a-GAL4	BDSC	Cat#41734; RRID:BDSC_41734
D. melanogaster: Ir76a-GAL4	BDSC	Cat#41735; RRID:BDSC_41735
D. melanogaster: GMR22C06-GAL4 (attP2)	BDSC	Cat#48974; RRID:BDSC_48974
D. melanogaster: GMR24G07-GAL4 (attP2)	BDSC	Cat#49095; RRID:BDSC_49095
D. melanogaster: GMR68D02-GAL4 (attP2)	BDSC	Cat#39471; RRID:BDSC_39471
D. melanogaster: GMR26B04-GAL4 (attP2)	BDSC	Cat#49158; RRID:BDSC_49158
D. melanogaster: GMR48E05-GAL4 (attP2)	BDSC	Cat#50370; RRID:BDSC_50370
D. melanogaster: GMR24A10-GAL4 (attP2)	BDSC	Cat#49059; RRID:BDSC_49059
D. melanogaster: GMR95C02-GAL4 (attP2)	BDSC	Cat#48431; RRID:BDSC_48431
D. melanogaster: NP6115-GAL4	DGGR	Cat#113851; RRID:DGGR_113851
D. melanogaster: NP3062-GAL4	DGGR	Cat#113083; RRID:DGGR_113083
D. melanogaster: NP5021-GAL4	DGGR	Cat#113545; RRID:DGGR_113545
D. melanogaster: NP5103-GAL4	DGGR	Cat#113584; RRID:DGGR_113584
D. melanogaster: NP80-GAL4	DGGR	Cat#103518; RRID:DGGR_103518
D. melanogaster: NP2001-GAL4	DGGR	Cat#104059; RRID:DGGR_104059
D. melanogaster: NP2297-GAL4	DGGR	Cat#112868; RRID:DGGR_112868
D. melanogaster: NP3529-GAL4	DGGR	Cat#113327; RRID:DGGR_113327
D. melanogaster: NP5288-GAL4	DGGR	Cat#104937; RRID:DGGR_104937
D. melanogaster: NP225-GAL4	DGGR	Cat#112095; RRID:DGGR_112095
D. melanogaster: NP1579-GAL4	DGGR	Cat#104036; RRID:DGGR_104036
D. melanogaster: Orco-GAL4	BDSC	Cat#26818; RRID:BDSC_26818
D. melanogaster: VT050660-GAL4 (attP2)	VDRC	Cat#200107
D. melanogaster: VT040053-GAL4 (attP2)	VDRC	Cat#201352
D. melanogaster: VT019428-GAL4 (attP2)	VDRC	Cat#200084
D. melanogaster: VT026020-GAL4 (attP2)	VDRC	Cat#203479
D. melanogaster: MZ612-GAL4	Gift from Dr. Liria Masuda-Nakagawa89,90	N/A
D. melanogaster: MB247-GAL4	BDSC	Cat#50742; RRID:BDSC_50742
D. melanogaster: c305a-GAL4	BDSC	Cat#30829; RRID:BDSC_30829
D. melanogaster: OK107-GAL4	BDSC	Cat#854; RRID:BDSC_854
D. melanogaster: LN1-GAL4	DGGR	Cat#103945; RRID:DGGR_103945
D. melanogaster: LN2-GAL4	DGGR	Cat#104198; RRID:DGGR_104198
D. melanogaster: MZ699-GAL4	Gift from Dr. Liqun Luo91	N/A
D. melanogaster: LC16-1-GAL4: R26A03-p65ADZp (attP40); R54A05-ZpGAL4DBD (attP2)	BDSC	Cat#68331; RRID:BDSC_68331
D. melanogaster: TLA-GAL4: VT012330-p65ADZp (attP40); VT020742-ZpGAL4DBD (attP2)	BDSC21,88	Cat#74205; RRID:BDSC_74205 Cat#72912; RRID:BDSC_72912
D. melanogaster: LC16-LexA: R28F07-LexAp65 (JK22C)	Gift from Dr. Barry J. Dickson20	N/A
D. melanogaster: MDN-LexA: UAS-CsChrimson-mCherry (attP5), VT044845-LexAp65 (attP40)	Gift from Dr. Barry J. Dickson20	N/A
Drosophila melanogaster: nSyb-IVS-PhiC31 (attP18)	BDSC	Cat#84151; RRID:BDSC_84151
D. melanogaster: SPARC2-D-CsChrimson::tdTomato	BDSC	Cat#84143; RRID:BDSC_84143
D. melanogaster: SPARC2-I-CsChrimson::tdTomato	BDSC	Cat#84144; RRID:BDSC_84144
D. melanogaster: SPARC2-S-CsChrimson::tdTomato	BDSC	Cat#84145; RRID:BDSC_84145
D. melanogaster: UAS-CsChrimson.mVenus (attP40)	BDSC	Cat#55135; RRID:BDSC_55135
D. melanogaster: UAS-CsChrimson.mVenus (attP2)	BDSC	Cat#55136; RRID:BDSC_55136
D. melanogaster: UAS-ChR2-XXM	Gift from Dr. Robert J. Kittel42	N/A
D. melanogaster: UAS-GtACR2.EYFP (attP2)	Gift from Dr. Adam Claridge-Chang72	Cat#92987; RRID:BDSC_92987
D. melanogaster: UAS-TNT	BDSC	Cat#28838; RRID:BDSC_28838
D. melanogaster: UAS-TNT inactive	BDSC	Cat#28839; RRID:BDSC_28839
D. melanogaster: UAS-FRT-STOP-FRT-CsChrimson.mVenus (attP2)	Gift from Dr. Galit Shohat-Ophir44	N/A
D. melanogaster: tub-FRT-STOP-FRT-GAL80	BDSC	Cat#39213; RRID:BDSC_39213
D. melanogaster: LexAop-CsChrimson.mVenus (attP40)	BDSC	Cat#55138; RRID:BDSC_55138
D. melanogaster: UAS-GCaMP6f (attP40)	BDSC	Cat#42747; RRID:BDSC_42747
D. melanogaster: UAS-GCaMP6f (VK00005)	BDSC	Cat#52869; RRID:BDSC_52869
D. melanogaster: LexAop-mCD8::GFP (attP2)	BDSC	Cat#32203; RRID:BDSC_32203
D. melanogaster: LexAop-GCaMP6m (attP1)	BDSC	Cat#44275; RRID:BDSC_44275
D. melanogaster: LexAop-GCaMP6m (VK00005)	BDSC	Cat#44276; RRID:BDSC_44276
D. melanogaster: Otd-nls:FLPo	Gift from Dr. Stephen F. Goodwin50	N/A
D. melanogaster: QUAS-GAL80	BDSC92	N/A
D. melanogaster: tsh-GAL80	Gift from Dr. Gero Miesenböck, and Dr. Christian Wegener15	N/A
D. melanogaster: LexAop-TNT::HA (VK00033)	Gift from Dr. Andrew Lin93	N/A
D. melanogaster: QUAS-CsChrimson.mVenus#32c	Gift from Dr. Christopher J. Potter	Cat#91994; RRID:BDSC_91994
D. melanogaster: Gr93a-GAL4	BDSC	Cat#57679; RRID:BDSC_57679
D. melanogaster: Gr89a-GAL4	BDSC	Cat#57676; RRID:BDSC_57676
D. melanogaster: Gr66a-GAL4	BDSC	Cat#57670; RRID:BDSC_57670
D. melanogaster: Gr39a.a-GAL4	BDSC	Cat#57630; RRID:BDSC_57630
D. melanogaster: Gr33a-GAL4	BDSC	Cat#57623; RRID:BDSC_57623
D. melanogaster: Gr32a-GAL4	BDSC	Cat#57622; RRID:BDSC_57622
Software and Algorithms
MATLAB	MathWorks	https://www.mathworks.com/products/matlab.html; RRID:SCR_001622
GraphPad Prism	GraphPad Software	https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798
Fiji	Schindelin et al.94	https://fiji.sc/; RRID:SCR_002285
neuPrint+	HHMI's Janelia Research Campus62	https://neuprint.janelia.org/?dataset=hemibrain:v1.2.1&qt=findneurons
FlyWire	Branson et al.95	https://flywire.ai/; RRID:SCR_019205
Natverse	Bates et al.61	https://natverse.org/
NeuronBridge	HHMI's Janelia Research Campus56	https://neuronbridge.janelia.org/
Amira	Thermo Fisher Scientific	https://www.thermofisher.com/il/en/home/electron-microscopy/products/software-em-3d-vis/amira-software.html; RRID:SCR_007353
Ctrax	Branson et al.95	http://ctrax.sourceforge.net/
MScan	Sutter Instrument	https://www.sutter.com/MICROSCOPES/mcs.html
LabVIEW	National Instruments	https://www.ni.com/en-il/shop/labview.html; RRID:SCR_014325
Pixelink Capture OEM	Pixelink	https://pixelink.com/products/software/pixelink-capture-software/pixelink-capture-software-download/
LAS AF	Leica Microsystems	N/A