Figure 6.

SMR4 slows down the cell-cycle progression of ACD through G1 extension

(A and D) Cell-cycle duration of ACD and SCD measured by PlaCCI in proPOLAR::SMR4 (A) and smr4-1cr (D). n = 15 for each cell division mode.

(B, C, E, and F) Representative time-lapse confocal images of ACD and SCD in stomatal lineage cells from 1 to 3-day-old cotyledon of proPOLAR::SMR4 (B and C) and smr4-1cr (E and F) expressing PlaCCI. Cell outlines (green) for proPOLAR::SMR4 (B and C) were hand-drawn based on digital overexposure of confocal images. LTi6B (green) are introduced into smr4-1cr (E and F). For the color code and time setting, see Figure 1 legends. White arrows indicate the nuclei with fluorescent signal. Pink arrows indicate the nucleus of a sister cell from the prior round of ACD. Scale bar, 10 μm.

(G and H) Cell-cycle duration of ACD (G) and SCD (H) among WT, smr4-1cr and proPOLAR::SMR4.

(I and J) G1 phase duration of ACD (I) and SCD (J) among WT, smr4-1cr, and proPOLAR::SMR4.

(A, D, G, H, I, and J) Two-tailed Student’s t test was performed. p values were indicated on top of each boxplot.

See also Table S1, and Videos S3 and S4.