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. 2021 Dec 15;45(3):112–121. doi: 10.14348/molcells.2021.0183

Fig. 1. CK2 upregulated and induced SIRT1 and AMPK activation during calorie restriction in human cancer cells.

Fig. 1

(A) HCT116 and MCF-7 cells were incubated under CR conditions for the indicated time. (B and C) Cells were transfected with CK2α siRNA for 1.5 days, then incubated under CR conditions for 7 h. The levels of each protein (A and B) and mRNA (C) were then determined by immunoblotting using specific antibodies, and by RT-PCR analysis using specific primers, respectively (top). Representative data from three independent experiments are shown. β-Actin was used as a control. The graphs represent the quantitation of CK2α, CK2β, and SIRT1 relative to β-actin, and p-AMPK (T172) relative to the unphosphorylated AMPK (bottom). Data were reported as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Bars that do not have the same letter (a, b, c) were significantly different among groups at P < 0.05.