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. 2021 Dec 15;45(3):112–121. doi: 10.14348/molcells.2021.0183

Fig. 2. CK2 positively regulated autophagy in human cancer cells.

Fig. 2

HCT116 and MCF-7 cells were transfected with CK2α siRNA or pcDNA3.1-HA-CK2α for 2 days. (A) Immunoblotting was then used to determine the level of each protein using specific antibodies (top). (B) The level of each mRNA was determined by RT-PCR using specific primers (top). Representative data from three independent experiments are shown. β-Actin was used as a control. The graphs represent quantitation of each protein and mRNA relative to β-actin (bottom). Data were reported as mean ± SEM. Bars that do not have the same letter (a, b, c) were significantly different among groups at P < 0.05.