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. 2021 Dec 15;45(3):112–121. doi: 10.14348/molcells.2021.0183

Fig. 3. CK2 positively regulated autophagy via mTOR and SIRT1 in human cancer cells.

Fig. 3

(A) HCT116 and MCF-7 cells were transfected with CK2α siRNA for 2 days in the absence or presence of 10 μM rapamycin or 10 μM resveratrol. The levels of each protein (left-top) and mRNA (left-bottom) were determined by immunoblotting using specific antibodies and by RT-PCR using specific primers, respectively. Representative data from three independent experiments are shown. β-Actin was used as a control. The graphs represent the quantitation of each protein (right-top) and mRNA (right-bottom) relative to β-actin. WB, Western blotting. (B) Cells were transfected with CK2α siRNA for 2 days in the absence or presence of pECE-flag-SIRT1 or pECE-flag-SIRT1 mutant (H363Y). The levels of each protein (left-top) and mRNA (left-bottom) were determined by immunoblotting using specific antibodies and by RT-PCR using specific primers, respectively. Representative data from three independent experiments are shown. β-Actin was used as a control. The graphs represent the quantitation of each protein (right-top) and mRNA (right-bottom) relative to β-actin. Data are reported as mean ± SEM. Bars that do not have the same letter (a, b, c) were significantly different among groups at P < 0.05.