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. 2021 Dec 15;45(3):112–121. doi: 10.14348/molcells.2021.0183

Fig. 6. Kin-10 RNAi decreased autophagy markers in nematodes.

Fig. 6

(A) Synchronized (L4 larva) reporter nematodes lgg-1::gfp were fed on control RNAi or kin-10 RNAi plates containing AICAR (100 μM), triciribin (TCN, 0.2 μM), or resveratrol (RSV, 50 μM) under standard conditions. Representative fluorescence images at day 1 of adulthood were obtained at 10× magnification (top). The fluorescence intensity of GFP was quantified using ImageJ software by determining the average pixel intensity (n = 30 per condition). (B) Synchronized (L4 larva) nematodes were fed on control RNAi or kin-10 RNAi plates containing AICAR (100 μM), triciribine (TCN, 0.2 μM), resveratrol (RSV, 50 μM), or spermine (SPM, 0.2 μM) under standard conditions. Subsequently, lysates from nematodes were used in RT-PCR using specific primers. The level of each mRNA was determined by RT-PCR (top). Representative data from the three independent experiments are shown. Act-2 was used as a control. The graphs represent the quantitation of each protein and mRNA relative to act-2 (bottom). Data are reported as mean ± SEM. Bars that do not have the same letter (a, b, c) were significantly different among groups at P < 0.05.