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. 2022 Mar 16;12(3):e759. doi: 10.1002/ctm2.759

FIGURE 4.

FIGURE 4

GO analysis reveals enrichment of proteins involved in RNA splicing, autophagy and lysosome, endoplasmic reticulum lumen and unfolded protein response and visual perception pathway in RP11‐retinal organoids. (A) GO analysis showing DE proteins between control and RP11 retinal organoids involved in RNA splicing, highlighting with black circle PRPF31 protein. (B) GO analysis showing DE proteins between control and RP11 retinal organoids involved in autophagy and lysosome. (C) Western blot showing the downregulation of PRPF31 protein in RP11 retinal organoids compared to unaffected (WT1) and isogenic control organoids. Western blot showing upregulation of LAMP1 and LAMP2 in RP11 retinal organoids compared to unaffected (WT1) and isogenic control organoids. Representative images from three independent experiments in retinal organoids at day 150 of differentiation are shown. ACTB was used as a loading control. GO analysis identifies DE proteins between RP11‐retinal and control organoids belonging to the (D) endoplasmic reticulum lumen, (E) unfolded protein response and (F) visual perception.