Figure 1.

Cross-sectional and longitudinal soluble TREM2 levels in CSF according to estimated years to symptom onset (EYO) in carriers and non-carriers of pathogenic variants

(A) Soluble TREM2 baseline levels plotted against EYO at baseline for carriers of pathogenic variants (shown in red, n=148) and non-carriers (shown in blue, n=91). The dotted line at –21 years indicates the timepoint at which cross-sectional soluble TREM2 levels start to be statistically higher in carriers of pathogenic variants than in non-carriers, according to the method described by McDade and colleagues.¹⁹ The dotted line at 0 years represents the expected point of symptom onset. Lines represent locally weighted scatterplot smoothing (LOESS) best-fitting curves (B) Spaghetti plot showing the longitudinal levels of soluble TREM2 in CSF from carriers of pathogenic variants (depicted by the red line, n=148) and non-carriers (depicted by the blue line, n=91) as a function of EYO. The dotted line at 0 years represents the expected point of symptom onset. Negative EYO values represent the expected presymptomatic phase. Positive values indicate the expected symptomatic phase of the disease. Because of the low number of participants located at the extremes of the graph, and to maintain their confidentiality, individual participants are not shown in the timeframe before –30 years and after 10 years.