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. 2022 Mar 3;10:851178. doi: 10.3389/fchem.2022.851178

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Copyright © 2022 Ma, Zheng, An and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative ratio of ECL intensity before (I₀) and after (I₀) incubation with buffer (blank), CEA (500 ng ml⁻¹), CA15-3 (500 ng ml⁻¹), AFP (500 ng ml⁻¹), PSA (0.1 ng ml⁻¹), or protein mixture (0.1 ng ml⁻¹ PSA + 500 ng ml⁻¹ other proteins).