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. 2022 Jan 12;126(6):937–947. doi: 10.1038/s41416-021-01621-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Definition of the TF and TC tumour areas. b Representative histomorphological images depicting laser-microdissected TF and TC areas as defined above. Only viable tumour areas were dissected. I. Overview of the different areas: TF and TC at ×25 magnification with the marked areas prior to LCM. In rows II. and III., the dissected areas are shown at ×25 and ×100 magnification. TF tumour invasion front, TC tumour centre.