Table 2.

Detected disulfide-linked peptides and experimental masses for representative batches of SB11 and EU/US-ranibizumab

Type of disulfide bond		Cysteine sites	Theoretical mass (Da)	Theoretical m/z (charge state)	Experimentally detected m/z
					SB11	EU-ranibizumab	US-ranibizumab
Heavy chain	Ha:Tb2–H:T9	Cys22–Cys96	3407.47	682.50	682.50	682.50	682.50
	H:T12–H:T13	Cys150–Cys206	7916.92	1132.00	1132.00	1132.00	1132.00
Light chain	Lc:T2–L:T5	Cys23–Cys88	7343.40	1469.69	1469.69	1469.69	1469.69
	L:T9–L:T16	Cys134–Cys194	3555.75	712.16	712.16	712.16	712.16
Interchain	H:T17–L:T18	Cys226–Cys214	756.24	757.25	757.25	757.25	757.25

^aHeavy chain

^bTryptic peptide

^cLight chain