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. 2022 Mar 3;10:806081. doi: 10.3389/fcell.2022.806081

FIGURE 9.

FIGURE 9

Dysregulation of Nrf2-GPx4/xCT pathway was related to ferroptosis induced by atorvastatin. (A) Ratio of GSH/GSSG in lysates from the cultured myocytes. (B) Glutathione peroxidase activity in lysates from the cultured myocytes. (C, D) Expression of proteins of Nrf2, SLC7A11, and GPx4 in cultured HCM treated by atorvastatin alone or with Fer-1 detected by Western blot. (E,F) Expression of proteins of Nrf2, SLC7A11, and GPx4 in cultured C2C12 detected by Western blot. (G,H) Expression of GPx4 protein in cultured HCM atorvastatin-treated alone or with Fer-1 in cytoplasm (without mitochondria) or mitochondria detected by Western blot. (I,J) Expression of GPx4 protein in cultured C2C12 atorvastatin-treated alone or with Fer-1 in cytoplasm (without mitochondria) or mitochondria in C2C12 detected by Western blot. The fluorescence intensity was quantified by ImageJ software. The data are shown as mean ± SD. ns, no significant; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the atorvastatin group. n = 3. Scale bar: 10 μm.