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. 2022 Mar 3;13:819699. doi: 10.3389/fgene.2022.819699

FIGURE 6.

FIGURE 6

MiRNA-105-5p in EVs can be transferred to ESCC cells. (A) Bar chart of miRNA-105-5p expression in different exosomes or microvesicles detected by the EVmiRNA database; (B) Transmission electron microscope was used to observe the forms of serum EVs of ESCC patients and normal persons (ratio: 100 nm); (C) Protein expression of EVs markers TSG101, CD9, and CD63 in serum EVs of ESCC patients and normal persons; (D) MiRNA-105-5p expression in serum EVs of ESCC patients and normal persons; (E) A fluorescence microscope was employed to detect whether serum EVs of ESCC patients could be internalized by ESCC cells: I. Alex Fluro594 phalloidin-labeled F-actin (red fluorescence); II. PKH67-labeled microvesicles (green fluorescence); III. DAPI-labeled cell nuclei (blue fluorescence); IV: Merge; Scale bar = 50 nm; (F) MiRNA-105-5p expression in ESCC cells after ESCC cells were co-cultured with serum EVs of ESCC patients. *p < 0.05.