Fig. 2.

Gene and protein expression of endothelial marker, tight junction proteins and transporters between HBEC (blue) and hiPS-BMEC (orange) (n ​= ​3). (A) RT-qPCR results. Data are presented as means ​± ​S.D. The biological replicates of HBEC and hiPS-BMEC come from three independent differentiations. (B) Immunostaining observed by confocal light scanning microscopy. Scale bar ​= ​150 ​μm. The images were taken using same exposure time and excitation power for each protein with HBEC and hiPS-BMEC. (C) Immunostaining fluorescence intensity comparison using pictures from Fig. 2B. Data are presented as means ​± ​S.D. Statistical analysis was performed using the Student's t-test (∗p ​≤ ​0.05, n. s. p ​> ​0.05). The biological replicates of HBEC and hiPS-BMEC come from three independent differentiations. (D) Zoom in pictures of immunostaining observed by confocal light scanning microscopy. The images were taken using different exposure time and excitation power for each sample. Scale bar ​= ​10 ​μm. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)