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. 2022 Mar 14;3(2):101245. doi: 10.1016/j.xpro.2022.101245
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, peptides, and recombinant proteins

Oxygen Airgas UN1072
Saline Hospira, Inc. NDC 0409-1966-02
Meloxicam Covetrus North America NDC 11695-6936-1
Isoflurane Covetrus North America NDC 11695-6777-2
Artificial tears ointment AKORN NDC 17478-062-35
Hair remover lotion Church & Dwight Co., Inc LL9023
70% ethanol VWR 89370-084
Povidone-iodine Purdue Products L.P. NDC 67618-154-16
Lidocaine hydrochloride Sigma-Aldrich 1366013
Bupivacaine hydrochloride Sigma-Aldrich 1078507
8% hydrogen peroxide The OneMinuteMiracle Inc. #7722-84-1
Dental cement (C&B Metabond) Parkell 375-0407
3M Vetbond tissue adhesive Amazon 1469SB

Experimental models:Organisms/strains

Female and male Pum1 mutant mice and wild type littermates in B6/129 mixed background; 12 weeks of age at surgery Prof. Haifan Lin’s Lab (Yale School of Medicine, New Haven, Connecticut 06520, USA) (Gennarino et al., 2018)

Software and algorithms

Data acquisition software Molecular Devices pCLAMP 10
ANY-maze behavioral tracking software Stoelting Version 6.30

Other

PFA-coated tungsten wire A-M Systems Cat. # 795500
PFA-coated silver wire A-M Systems Cat. # 786000
Stainless steel tubing, 30 Gauge A-M Systems Cat. # 832000
Sandpaper 3M 400 Grit
Solder flux MG Chemicals FLUX H150
Nylon surgical suture AD Surgical #S-N518R13
Male connector Harwin M22-2580405
Female connector Harwin M22-7140442
USB camera Imaginesource DMK 22AUC03
Camera lens FUJINON YV5x2.7R4B-2
Dissection microscope A-M Systems EMZ-5TR
Stereo microscope ZEISS Stemi 2000-C
Soldering station Weller Model WES51
Multimeter Fluke Fluke 179
0.5 mL insulin syringes ADW Diabetes # SY8290328279
Isoflurane vaporizer Veterinary Anesthesia Systems, Inc. Matrx VIP 3000
Fiber optic illuminator A-M Systems 725910
Small animal stereotaxic instrument David Kopf Model 962
Glass bead sterilizer Cellpoint Scientific, Inc. 5-1450
Homeothermic blanket system Harvard Apparatus 507220F
Animal fur trimmer WAHL #9861-900
High speed drill The Foredom Electric Company Model 1474
Heat therapy pump Gaymar Industries, Inc. Model # TP650
Field potential recording chamber Pinnacle Technology Inc. 8228
Faraday cage Technical Manufacturing Corporation No.81-344-04
4-Channel preamplifier Pinnacle Technology Inc. 8406-SE4
8-Channel mouse commutator Pinnacle Technology Inc. 8408
Differential AC amplifier A-M Systems Model 1700
Digitizer Molecular Devices 1440A
Batteries Amazon Size AA
Digital interface for ANY-maze Stoelting AMi-2
PC computer Dell 64-bit, Windows 10
Surgical tools: fine scissors, scalpel, fine forceps, coarse forceps, 0.7 mm burs Fine Science Tools https://www.finescience.com/en-US/
Straight fissure crosscut bur Dentalaire https://www.dentalaireproducts.com/products/burs-and-diamond-discs/