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Chromatographic separation of two HexNAc isomers. The two HexNAc standards (A) GlcNAc and (B) GalNAc were 2-AB-labeled and analyzed separately. (C) Both species were found in the HEK293WT total cell lysate. Idotp, isotopic dot product between theoretical and observed isotopic pattern. The blue, purple, and red lines correspond to the extracted ion chromatograms of the mono-isotopic mass, the second isotopologue, and the third isotopologue, respectively.
