Table 1. Primer Sequences Used for qRT-PCR Gene Expression Analysis.
genes | 5′-3′ | primer sequences |
---|---|---|
GAPDH | sense | 5′-AAATGGTGAAGGTCGGTGTGAAC-3′ |
antisense | 5′-CAACAATCTCCACTTTGCCACTG-3′ | |
RUNX2 | sense | 5′-TGCAAGCAGTATTTACAACAGAGG-3′ |
antisense | 5′-GGCTCACGTCGCTCATCTT-3′ | |
COL-I | sense | 5′-GACATGTTCAGCTTTGTGGACCTC-3′ |
antisense | 5′-GGGACCCTTAGGCCATTGTGTA-3′ | |
ALP | sense | 5′-GCAGTATGAATTGAATCGGAACAAC-3′ |
antisense | 5′-ATGGCCTGGTCCATCTCCAC-3′ | |
OPN | sense | 5′-TACGACCATGAGATTGGCAGTGA-3′ |
antisense | 5′-TATAGGATCTGGGTGCAGGCTGTAA-3′ | |
OCN | sense | 5′-AGCAGCTTGGCCCAGACCTA-3′ |
antisense | 5′-TAGCGCCGGAGTCTGTTCACTAC-3′ |