TABLE 6.

A cost analysis of MiSeq, Mk1B, and Mk1C sequencing using various flow-cell loads.

= Illumina Technology; = Oxford Nanopore Technology; = Nvidia Technology All pricing in GBP (£) as of August 2021. The precision protocol entails halving reagent volumes from the Nextera library preparation kit’s manufacturer-provided SOP and reducing SPRI beads from 22.5 to 11.5 μl/sample. Among 6 suppliers and 17 models of filter tips covering various ranges within 0.5–200 μl, only 4 models (Appleton Woods ACA659, ACT659, ACT619, and Starlabs S1120-3810) yielded DNA concentrations sufficiently conformant to carry on to sequencing with this method (data not shown). Our groups have not yet attempted to create a similarly reagent-optimized ONT protocol.