Figure 7.

Glycolysis, the P cycle, electron transport chain with and without exogenous glucose in PA-R8_SCF and PA-R16_SCF. (A) Metabolic diagram of glycolysis, the P cycle, electron transport chain. (B) qRT-PCR for expression of genes encoding glycolysis and the P cycle between PA-R8_SCF, PA-R16_SCF, and PA-S8, PA-S16, respectively. (C) Activity of enzymes in glycolysis, the P cycle, electron transport chain between PA-R8_SCF, PA-R16_SCF, and PA-S8, PA-S16, respectively, in the presence or absence of different concentrations of glucose. (D) qRT-PCR for expression of genes encoding glycolysis and the P cycle between PA-R8_SCF, PA-R16_SCF, and PA-S8, PA-S16, respectively, in the presence of different concentrations of glucose. (E–H) NADH (E), membrane potential (F), ATP (G), and amikacin (H) of the two PA-R and three PA-S strains in the presence or absence of glucose. Results are displayed as mean ± SEM, and at least three biological repeats are performed. Significant differences are identified ^*p < 0.05, ^**p < 0.01.