Table 3.

List of selected mutants in the wheat DRE2 genes showing reductions (in percent) or over accumulations in the content of various gluten proteins.

Mutant ID	Glutenin subunits		Gliadins				TKW (g)
	HMW	LMW	ω - Gliadins	ω-D Gliadins	γ - Gliadins	α/β - gliadins
Dre2-2AL
SNV Kronos2205:G9670A	1.3 times	13	67	39	78	2.2 times	46.7
SNV Kronos604:G10513A	63	44	2.9 times	Missing	65	1.2 times	30.0
SNV Kronos2630:C9648T	64	34	2 times	1.9 times	27	1.2 times	50.0
SNV Kronos2084:G9912A	78	33	83	39	71	2.7 times	50.0
SNV Kronos601:G10126A	58	39	78	46	75	2.2 times	40.0
SNV Kronos2189:C10139T	36	24	91	59	83	1.9 times	30.0
SNV Kronos974:C10142T	73	6	Missing	Missing	90	2.3 times	70.0
SNV Kronos2312:C10190T	1.5 times	1.2 times	Missing	77	86	1.6 times	46.7
SNV Kronos382:G10304A	0	1.1 times	27	30	40	14	30.0
SNV Kronos3076:C10497T	14	1.1 times	Missing	68	84	1.3 times	30.0
SNV Kronos563:C10510T	5.5 times	1.4 times	67	34	53	1.6 times	43.3
SNV Kronos1214:G10600A	1.9 times	1.1 times	Missing	74	84	1.4 times	36.7
SNV Kronos799:C12741T	1.5 times	1.2 times	Missing	67	80	1.9 times	43.3
Dre2-2BL
SNV Kronos2719:G3923A	1.8 times	5	2.1 times	1.2 times	3	20	33.3
SNV Kronos1061:G4033A	1.5 time	2.2 times	Missing	87	88	1.6 times	36.7
SNV Kronos2225:G4039A	3.8 times	1.8 times	Missing	94	93	1.1 times	33.3
“Kronos”	0	0	0	0	0	0

Missing, missing protein band(s).

Fold increase in the gluten protein content is shown in times.

TKW, thousand-kernel weight. It is extrapolated from the weight of 100 kernels.

The proteins were resolved via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and their content was determined compared to the bovine serum albumin (BSA).