FIG. 1.

Effect of medium on trailing. The susceptibility of isolate 707-15 to fluconazole was tested in RPMI 1640 (solid symbols) and YAD (open symbols), using the microtiter version of the M27-A method, as described in the text. Relative growth (y axis) is calculated as the percentage of growth (optical density at 530 nm) relative to growth in a drug-free well (Growth Control) for serial dilutions of fluconazole (x axis). The squares represent 48-h readings, and the circles represent 24-h readings of the same plate. The results represent the means from experiments performed in triplicate.