FIG. 3.

pH arrays in RPMI 1640 and YAD. C. albicans trailing isolate 707-15 was used to assess trailing in RPMI 1640 (A) and YAD (B) adjusted to pHs 7 (solid squares), 6.5 (solid diamonds), 6.0 (solid triangles), 5.5 (solid circles), 5.0 (open triangles), 4.5 (open squares), and 4.0 (open diamonds). All media were buffered in MOPS (0.165 M) and incubated with shaking at 33°C. Relative growth (y axis) is calculated as the percentage of growth (optical density at 530 nm) relative to growth in a drug-free well (Growth Control), for serial dilutions of fluconazole (x axis). Shown are the results of growth after 48 h.